Journal: International Journal of Medical Sciences

Article Title: Naringenin exerts anticancer effects by inducing tumor cell death and inhibiting angiogenesis in malignant melanoma

doi: 10.7150/ijms.44804

Figure Lengend Snippet: Effect of naringenin treatment on ERK1/2 and JNK MAPKs signaling. (A-D) Images and quantification of protein expression of p-ERK1/2 and p-JNK in B16F10 (A, B) and SK-MEL-28 cells (C, D) after naringenin treatment in a dose-dependent manner. Values are mean ± SD from ≥ three independent experiments. * p = 0.0265, ** p < 0.0071, *** p = 0.0006; ## p = 0.0013, ### p = 0.0002 versus untreated cells by one-way ANOVA followed by Bonferroni post-tests.

Article Snippet: After blocking with 5% skim milk, the membranes were incubated with the following primary antibodies in blocking buffer overnight at 4 °C: anti-caspase-3 (Cas3) (rabbit), anti-Poly (ADP-ribose) polymerase (PARP), phosphorylated extracellular signal-regulated kinases1/2 (p-ERK1/2) (rabbit), andphosphorylated c-Jnk N-terminal kinase (p-JNK) (mouse) (all from Cell Signaling Technology, Inc., Beverly, Ma, USA)and anti-β-actin (mouse monoclonal; Sigma-Aldrich).

Techniques: Expressing